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AAPPTec
 

Antibodies
  1. What is the difference between an epitope and a paratope?

  2. What is an idiotope?

  3. What is a hapten?

  4. How should I store antibodies?

  5. Do the impurities in my peptide affect the antibodies that are produced?


  1. What is the difference between an epitope and a paratope?


    An epitope is the part of an antigen that and antibody binds to.  A paratope is the part of an antybody that binds to an antigen.  Paratopes are on the ends of the arms of antibodies.  Epitopes are on the surface of antigens.  



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  2. What is an idiotope?


    An idiotope is an antigenic determinant on the light chain of an antibody.  The idiotope contains the paratope which is the portion of the antibody that physically binds to the epitopes of the antigen.



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  3. What is a hapten?


    A hapten is a molecule that is the desired target for antibodies, but is too small to cause an immune response.  A synthetic peptide, for instance, is a hapten.  By itself, it will not elicit an immune response.  It must be conjugated to a carrier protein in order to promote antibody production.



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  4. How should I store antibodies?


    Antibodies should be stored frozen at -20 C.  Avoid thawing and refreezing antibody solutions.  



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  5. Do the impurities in my peptide affect the antibodies that are produced?


    Generally, no.  A peptide that is only 80% pure is much more abundant than any of the impurity peptides.  Therefore the antibodies antibodies specific to the peptide are more likely form than t



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